1Department of Dermatology and Venereology, The 3rd Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China. 2Skin Research Center, Landproof Testing Technology Co., Ltd, Guangzhou, Guangdong 510635, China. 3Laboratory of Dermatology and Venereology, The 3rd Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China.

��Abstract�� Objective This study was to determine the antimicrobial susceptibility of P. acnes in acne patients in Guangzhou city of southern China. Methods Among 124 patients, we isolated 100 stains of P. acnes and did the antimicrobial susceptibility tests in vitro. Results By applying the Clinical and Laboratory Standards Institute document M11-A7 breakpoints for resistance, 11%, 39%, 36%, 71%, 72%, 53%, 3%, 28% of the stains were resistant to minocycline, doxycycline, tetracycline, erythromycin, azithromycin, clindamycin, ceftriaxone and levofloxacin, respectively. Moreover, severe cross/multiple resistance was also observed. Conclusion The high prevalence of antimicrobial resistant P. acnes should cause alarm and attention in Guangzhou.