1Department of Dermatology, Henan Provincial People′s Hospital (People′s Hospital of Zhengzhou University), Zhengzhou, Henan 450003, China. 2Department of Dermatology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu 210042, China. 3Department of Dermatology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China.

��Abstract�� Objective To describe the patterns of immunofluorescence mapping (IFM) in different forms of epidermolysis bullosa (EB) and classify the diagnosis of EB patients. Methods We used tissue specimens from six outpatients with congenital EB, which were made into frozen sections and paraffin sections, and stained for keratin 14 (K14), type IV collagen and type VII collagen using immunofluorescence and immunohistochemistry, simultaneously using normal skin as controls. Analysis was performed to determine which parts of the blister were labeled with the corresponding antibodies, and subtyping was performed on EB patients. Results The result showed that two cases of EBS and three cases of DEB were confirmed by IFM, while only one case of EBS and two cases of DEB were confirmed by immunohistochemistry. Conclusion IFM is an important method for subtyping EB patients, and it is superior to immunohistochemistry.