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| PubMed | |
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Article by XU Jing |
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Article by LUO Dan |
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�쾧, �浤
�Ͼ�ҽ�ƴ�ѧ��һ����ҽԺƤ���� 210029
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Application of Comparative Genomic Hybridization Technique in the Diagnosis of Cutaneous Tumors
Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
The comparative genomic hybridization (CGH) technique, based on the combination of fluorescence in situ hybridization (FISH) and a pruning technique, is developed as a molecular genetic method to analyze the gene insertion and deletion comprehensively and swiftly. By analyzing altofrequent chromosome mutations, CGH can provide locating information for screening susceptibility genes of cutaneous tumors. Specific results of CGH may conduce to the diagnosis and differential diagnosis of cutaneous tumors, assist in determining their types and stages as well as in evaluating their prognosis, and give clues to their occurrence and development.
[2] Bastian BC,LeBoit PE,Pinkel D.Genomic approaches to skin cancer diagnosis.Arch Dermatol,2001,137:1507-1511.
[3] Bastian BC.Molecular cytogenetics as a diagnostic tool for typing melanocytic rumors.Recent Results Cancer Res,2002,160:92-99.
[4] Bastian BC,Olshen AB,LeBoit PE,et al.Classifying melanocytic tumors based on DNA copy number changes.Am J Pathol,2003,163:1765-1770.
[5] Bastian BC.Understanding the progression of melanocytic neoplasia using genomic analysis:from fields to cancer.Oncogene,2003,22:3081-3086.
[6] Takata M,Maruo K,Kageshita T,et al.Two cases of unusual acral melanocytic tumors:illustration of molecular cytogenetics as a diagnostic tool.Hum Pathol,2003,34:89-92.
[7] Mao X,Lillington D,Child F,et al.Comparative genomic hybridization analysis of primary cutaneous B-cell lymphomas:identification of common genomic alterations in disease pathogenesis.Genes Chromosomes Cancer,2002,35:144-155.
[8] Mao X,Orchard G,Lillington DM,et al.Genetic alterations in primary cutaneous CD30+ anaplastic large cell lymphoma.Genes Chromosomes Cancer,2003,37:176-185.
[9] Mao X,Lillington DM,Czepulkowski B,et al.Molecular cytogenetic characterization of Sezary syndrome.Genes Chromosomes Cancer,2003,36:250-260.
[10] Bastian BC,Kashani-Sabet M,Hamm H,et al.Gene amplifications characterize acral melanoma and permit the detection of occult tumor cells in the surrounding skin.Cancer Res,2000,60:1968-1973.
[11] Fischer TC,Gellrich S,Muche JM,et al.Genomic aberrations and survival in cutaneous T cell lymphomas.J Invest Dermatol,2004,122:579-586.
[12] Hallermann C,Kaune KM,Siebert R,et al.Chromosomal aberration patterns differ in subtypes of primary cutaneous B cell lymphomas.J Invest Dermatol,2004,122:1495-1502.
[13] Shimizu T,Izumi H,Oga A,et al.Epidermal growth factor receptor overexpression and genetic aberrations in metastatic squamouscell carcinoma of the skin.Dermatology,2001,202:203-206.
[14] Bastian BC,Xiong J,Frieden IJ,et al.Genetic changes in neoplasms arising in congenital melanocytic nevi:differences between nodular proliferations and melanomas.Am J Pathol,2002,161:1163-1169.
[15] Wessendorf S,Fritz B,Wrobel G,et al.Automated screening for genomic imbalances using matrix-based comparative genomic hybridization.Lab Invest,2002,82:47-60.
[16] Heiskanen MA,Bittner ML,Chen Y,et al.Detection of gene amplification by genomic hybridization to cDNA microarrays.Cancer Res,2000,60:799-802.