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| PubMed | |
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Article by LIU Hong-fang |
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Article by XI Li-yan |
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Application of Differential Display Reverse Transcription-PCR in Medical Mycology
Department of Dermatology, the Second Affiliated Hospital, Zhongshan University, Guangzhou 510120, China
Department of Dermatology, the Second Affiliated Hospital, Zhongshan University, Guangzhou 510120, China
mRNA differential display reverse transcription-PCR (DDRT-PCR) is a technique to study the difference between gene expression at the level of transcription. It has become an efficient tool for molecular biology research of medically important fungi. Some genes related to the pathogenicity of Candida albi-cans, Aspergillus and other fungi have been found by this technique. It will help to understand the pathogenesis of systemic mycoses, to develop new antifungal agents, to prepare fungal vaccines and to make early diagnosis and treatment of mycoses.
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