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Ŀ�� ̽�ַ�����ŧ������м����GPP ���ķ�����׽��أ�IL��-1��-6�������������ӣ�TNF��-α�Ĺ�ϵ������ ����˫������� ABC-ELISA����ⷢ�����벻�����黼��Ѫ��IL-1��IL-6��TNF-α��ˮƽ����� GPP����������GPP�������س̶����֣�r = 0.858)����ϸ��������r = 0.429����������ϸ��������r = 0.518)��Ѫ��IL-1��r = 0.518����IL-6��r = 0.484)��TNF-α��r = 0.587��ˮƽ��������أ�P < 0.05��P < 0.01����GPP�����鷢��ʱѪ��IL-1��IL-6��TNF-αˮƽ������GPP����������ƽ��ʱѪ��IL-1��t = 3.639����IL-6��t′ = 2.276����TNF-α��t = 2.880��ˮƽ��GPP�������ԺʱѪ��IL-1��t = 5.129����IL-6��t = 3.348����TNF-α��t = 4.775��ˮƽ��GPP����������ԺʱѪ��IL-1��t = 2.154����IL-6��t′= 2.476����TNF-α��t′= 2.582��ˮƽ��GPP���������ԺʱѪ��IL-1��t = 3.212����IL-6��t′= 3.309����TNF-α��t = 4.566��ˮƽ�ͽ���������Ѫ��IL-1��t = 4.768����IL-6��t = 4.539����TNF-α��t = 8.478��ˮƽ��P < 0.05��P < 0.01����GPP���߸�������Ѫ��IL-1ˮƽ����IL-6ˮƽ��r = 0.575����TNF-αˮƽ��r = 0.790��������������أ�P ֵ�� < 0.01����GPP����Ѫ��IL-6ˮƽ��TNF-αˮƽ�����������أ�r = 0.579, P < 0.01�������� IL-1��IL-6��TNF-α����ϸ�����Ӽ���໥���ÿ�����GPP���߷��ȵ�����ѧ����֮һ��������ϸ����GPP���߷�������һ�������á�

Objective To investigate the relationship of fever with interleukin ��IL-1, -6 and tumor necrosis factor ��TNF-α in generalized pustular psoriasis ��GPP��. Methods Venous blood samples were obtained from 20 healthy controls, 24 patients with fever-associated GPP, 10 patients with fever-unassociated GPP at different time points. Serum levels of IL-1, IL-6 and TNF-α were determined by double-antibody sandwich enzyme-linked immunosorbent assay using an avidin-biotin peroxidase complex system ��ABC-ELISA��. Results The body temperature positively correlated with disease severity score ��r = 0.858, P < 0.01��, white blood cell count ��r = 0.429, P < 0.05��, neutrophil cell count ��r = 0.518, P < 0.05��, and serum levels of IL-1 ��r = 0.518, P < 0.05��, IL-6 ��r = 0.484, P < 0.05�� and TNF-α ��r = 0.587, P < 0.01�� in patients with GPP. The serum levels of IL-1, IL-6 and TNF-α in patients with GPP during the period of fever were significantly higher than those in patients with fever-associated GPP at normal temperature ��t = 3.639, 2.276, 2.880, P < 0.01 or 0.05�� and at the time of discharge ��t = 5.129, 3.348, 4.775, all P < 0.01��, those in patients with fever-unassociated GPP at the time of admission ��t = 2.154, 2.476, 2.582, all P < 0.05�� and discharge ��t = 3.212, 3.309, 4.566, all P < 0.01��, and those in healthy controls ��t = 4.768, 4.539, 8.478, all P < 0.01��. The serum level of IL-1 was positively correlated with that of IL-6 ��r = 0.575, P < 0.01�� and TNF-α ��r = 0.790, P < 0.01��, and the serum level of IL-6 was positively correlated with that of TNF-α ��r = 0.579, P < 0.01��. Conclusions The interaction between IL-1, IL-6 and TNF-α may be involved in the immunological mechanism of fever in GPP, and neutrophils may be associated with the fever in GPP.