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Objective To evaluate the performance of simultaneous amplification and testing ��SAT�� kit for detecting Neisseria gonorrhoeae��NG�� from swab and urine specimens. Methods A total of 205 clinical samples of male urethral swab, female endocervical swab and FCU��first-catch urine�� were collected from symptomatic and asymptomatic STD clinic clients. The swab samples were tested by both SAT and culture, while FCU samples by SAT only. The samples with inconsistent test results were further re-tested by another PCR. Results NG-SAT yielded the sensitivity and specificity of 100% and 94.5%, respectively, for both swab and urine samples. Six of 7 specimens with divergent results between SAT and culture showed consistent results between SAT and PCR. Conclusion These results indicate that SAT is sensitive, specific, and time-sparing in the detection of NG, which may provide a novel approach to the laboratory diagnosis of gonorrhea.